Objective:To explore the 5-HT mechanism of stress.Methods:The method of polymerase chain reaction(PCR)was used to detect the presence of 5-HT receptor subtypes in the hippocampus,hypothalamic and midbrain of the rat after 30days of movement restrict compared with normal cage control groups(CC). The specific oligonucleotide primers were synthesized based on the complementary DNA sequence for each of the rat 5-HT 1A and 5-HT _2A receptor subtypes.Results: The expression of 5-HT_1A receptor subtypes in each regions of the rat brain after movement restrict were signifcantly lower than that of the CC groups, and the expression of 5-HT_2A receptor subtype were signifcantly higher（P<0.01-0.05).Conclusions: 5-HT_1A and 5-HT _2A receptors may be invovled in the stress of the rat and may have opposite effects with respect to certain functions.

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