中国临床心理学杂志

创伤后应激障碍大鼠模型的情景恐惧记忆神经机制的研究

作者：陶嵘¹ 张亚林² 王芙蓉³ 向慧²

单位：1. 华中师范大学心理学院湖北武汉 430079
2. 中南大学湘雅二医院精神卫生研究所湖南长沙 410011
3. 国防科学技术大学军事心理学研究室

分类号：R395.1

出版年,卷(期):页码：2007,15(3):314-317

摘要：

目的：研究恐惧记忆时动物杏仁核胃泌素释放肽(gastrin-releasing peptide，GRP)的变化，以及地西泮对其的影响。方法：雄性SD大鼠随机分为5组，每组6只。条件恐惧训练后，分别于1h、1d后测定给予或不给予地西泮鼠的情景恐惧记忆水平和杏仁核GRP水平。结果：①训练组大鼠对恐惧情景的记忆水平明显高于对照组。②条件恐惧训练后GRP mRNA和蛋白水平显著升高，同时GRP与僵立行为之间存在显著负相关关系。③地西泮作用时GRP水平显著下降。结论：GRP及其与GABA的作用可能是一种恐惧记忆的负反馈调节因子，是一种值得探索的治疗PTSD新途径。地西泮对恐惧记忆的复杂作用可能与GRP有关。

Objective:To explore:①The change of gastrin-releasing peptide mRNA and protein during fear conditioning, and the correlation between this change and fear memory.②The interaction between GRP and Diazepam during fear memory.Methods:30 male SD rats were randomly assigned to 5 groups.After fear-conditioning training, retention tests were conducted to rats treated with or without diazepam either 30 minutes or 24 hours after training.Then the amygadala of the animals were isolated, semi-quantified RT-PCR were applied to measure GRP mRNA level, and semi-quantified western-bolt were applied to determine GRP protein level.Results:①Fear conditioning training could significantly increase the memory of conditioning context both in the 30 min and 24h groups.②Fear conditioning training could significantly increase the level of GRP mRNA and protein both in the 30 min and 24h groups, and there were significant correlation between freezing and GRP mRNA and protein.③GRP level decreased significantly when treated with diazepam.Conclusion:GRP may be a feedback factor of fear memory, it may be valuable in treating PTSD.Diazempam's complicated role for fear memory may have something to do with GRP.

基金项目：

作者简介：

参考文献：

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