中国临床心理学杂志

不同应激方式对模拟高原环境下大鼠海马单胺递质的影响

作者：马文涛邓自和杨来启何明吴兴曲张彦张建侠

关键词：应激低氧海马推挽灌流单胺递质

分类号：R363

出版年,卷(期):页码：2008,16(5):492-494

摘要：

目的：探讨心理和生理应激对模拟高原低压低氧环境下的大鼠海马单胺递质含量的影响。方法：雄性SD大鼠随机分为对照组(Con组)、低氧组(Hy组)、心理应激(2次/d)组(Ps1组)、心理应激(2次/d×2天)组(Ps2组)、心理应激(2次/d)+低氧组(Ps1+Hy组)、心理应激(2次/d×2天)+低氧组(Ps2+Hy组)、生理应激(2次/d)组(Ph1组)、生理应激(2次/d×2天)组(Ph2组)、生理应激(2次/d)+低氧组(Ph1+Hy组)和生理应激(2次/d×2天)+低氧组(Ph2+Hy组)。心理和生理应激都采用旁观电击(CB)的方法。用高效液相色谱-电化学检测法(HPLC-ECD)测定用推挽灌流收集的海马灌流液中的去甲肾上腺素(NE)，多巴胺(DA)和5-羟色胺(5-HT)的含量。结果：①与Con组比较，Hy、Ps1+Hy和Ph2+Hy组的NE含量均显著降低(P<0.01，0.05和0.01)；与Hy组比较，Ps2+Hy和Ph1+Hy组的NE含量均显著增高(P<0.01和0.05)。②与Con和Hy组比较，Ph1+Hy组的5-HT含量均显著升高(P<0.01和0.05)。③与Con和Hy组比较，Ph1+Hy组的DA含量均显著升高(P<0.01)。结论：心理和生理应激皆能显著影响低压低氧环境下大鼠海马单胺递质的含量。

Objective:To examine the effects of psychological and physiological stress on hippocampal extracellular?monoamine content under simulated high-altitude hypobaric hypoxia.Methods:The male SD rats were randomly divided?into ten groups:control(Con group),hypoxia (Hy group),psychological stress (twice/day) (Ps1group),psychological stress?(twice/day×2day) (Ps2 group),psychological stress (twice/day) + hypoxia(Ps1+Hy group),psychological stress (twice/day ×?2day) +hypoxia (Ps2+Hy group),physiological stress (twice/day) (Ph1group),physiological stress (twice/day×2day) (Ph2?group),physiological stress(twice/day) + hypoxia(Ph1+Hy group),physiological stress(twice/day×2day) + hypoxia(Ph2+Hy?group).The contents of norepinephrine( NE),dopamine (DA) and 5-hydroxytryptamine(5-HT) in extracellular fluid of hippocampus?collected by push-pull perfusion were determined by high performance liquid chromatography with electrichemical?detection (HPLC-ECD).Results:①Compared with the Con group,the content of NE was significantly lower in Hy,Ps1+Hy and Ph2+Hy groups (P<0.01,0.05,0.01,respectively);Compared with the Hy group,it was significantly higher in?Ps2+Hy and Ph1+Hy groups (P<0.01,0.05,respectively).②Compared with the Con and Hy group,the content of 5-HT?was significantly higher in Ph1+Hy group (P<0.01,0.05,respectively).③Compared with the Con and Hy group,the content?of DA in extracellular fluid of hippocampus was significantly higher in Ph1+Hy group( P<0.01).Conclusion:Psychological?and physiological stress had significant influence on hippocampal monoamine content in rat brain.

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参考文献：

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