中国临床心理学杂志

睡眠剥夺对大鼠一氧化氮和一氧化氮合酶的影响

分类号：B845.1

出版年,卷(期):页码：2002,10(2):106-107

摘要：

目的:探讨睡眠剥夺对大鼠脑组织一氧化氮(NO)及一氧化氮合酶(NOS)影响。方法:采用小平台水环境法(FlowerPot)制作大鼠睡眠剥夺模型,采用化学法和酶法观察不同时间睡眠剥夺后大鼠额叶、海马、中脑和下丘脑NO含量及NOS活性变化。结果:与正常对照组及大平台组比较,大鼠在SD后额叶和海马的NO含量及NOS活性增高,有显著性差异(P＜0.01～0.05),其余脑区无显著性差异(P＞0.05)。随着剥夺时间的延长,额叶和海马NO含量及NOS活性增高更加明显。结论:睡眠剥夺可致NO及NOS升高,可能与其学习障碍有关,NO可能参与大鼠的睡眠调节

Objective:To explore the brain levels of nitric oxide (NO) and nitric oxide synthase(NOS) in rats after sleep deprivation.Methods:NO levels and NOS activity were assayed in different brain regions of Sprague-Dawley rats after sleep deprivation by flower pot compare with normal group (CC) and tank control groups (TC).Results:NO levels and NOS activity were increased in front cortex and hippocampus after SD compared with CC and TC (P＜0.01～0.05).Conclusion:The NO levels and activity of NOS are increased after sleep deprivation,NOS/NO may play an important role in learning and memory and modulating sleep.

基金项目：

作者简介：

参考文献：

[1] 宋国萍,皇甫恩,苗丹民,等.睡眠剥夺对大鼠学习和行为的影响.第四军医大学学报,2000,21(6):663-666
[2] Chen J,Zhang S,Zuo P,et al.Memory-related changes of nitric oxide synthase activity and nitrite level in rat brain.Neuroreport,1997,8(7):1771-1774
[3] 刘辉,陈俊抛,田时雨,等.神经元型一氧化氮合酶在学习记忆过程中的变化和作用.中国神经免疫学和神经病学杂志,2000,17:116-122
[4] Bradley DY,Zhou J,Smagin GN,et al.Sleep deprivation by the "Flower Pot"technique and spatial reference memory.Physiol-Behav,1997,61:249-256
[5] Yamada K.Role of nitric oxide in learing and memory processes.Nippon Yakuriga kuzasshi,1998,111:87-96
[6] Holscher C.Nitric oxide the enigmatic neuronal messenger:its rode in synaptic plasticity.Trends Neurosci,1997,20(7):298-303
[7] 王景华,杨贵贞.一氧化氮对大鼠学习功能的影响.中国神经免疫学和神经病学杂志,1997,4(1):11-15
[8] Ward L,Mason SE,Abraham WC.Effects of the NMDA antagonists CPP and MK-801 on radial arm maze performance in rat.Pharmacol Biochem Behav,1990,35(4):785-790
[9] 田恒力,张镛.一氧化氮生物作用的研究进展.国外医学神经病学神经外科学分册,1995,22(2):87-90
[10] Molina JA,Jimenez JFJ,Orti PM,et al.The role of nitric oxide in neurodegen eraion Portential for pharmacologicl intervention.Drugs Aging,1998,12(4):251-259
[11] 张文慧,孙碧英,林殷利.NO对大鼠睡眠-觉醒的调节.中国应用生理学杂志,2000,16(4):339-342
[12] Kuriyama K,Ohkuma S.Role of nitric oxide in central synaptic transmitter rel ease.J Pharmzcol(Japan),1995,69:1.

服务与反馈：

【文章下载】【加入收藏】