中国临床心理学杂志

慢性应激对大鼠海马Bcl-xl表达的影响及应激后的变化

作者：毕波王哲彭淼金魁和韩继阳朱宇章

关键词：慢性应激海马 Bcl-xl 强迫游泳

分类号：R395.1

出版年,卷(期):页码：2005,13(2):144-146

摘要：

目的:探讨慢性应激对大鼠海马神经元Bcl-xl蛋白表达的影响及其应激后的变化。方法:采用慢性强迫冰水游泳制作动物模型。运用open-field法观察大鼠行为学的变化,运用免疫组织化学方法观察大鼠海马DG区、CA3区Bcl-xl的变化。结果:与对照组相比,实验组1大鼠海马CA3区齿状回(DG)区Bcl-xl平均灰度值显著增加(t=4.69,P<0.05和t=3.77,P<0.01),实验组2平均灰度值与对照组2相比同样增加(t=3.35,P<0.05和t=3.30,P<0.05)。结论:慢性应激使大鼠海马Bcl-xl表达降低,应激三十天后,其表达仍低于对照组。

Objective: To observe the changes of Bcl-xl expression in hippocampus of the rats with the forced-swimming and the changes of Bcl-xl after poststress. Methods: The protocol was established with the forced-swimming as the chronic stress model. Open-field test was executed to measure the behaviors of the rats. Immunohistochemistry was used to observe the changes of Bcl-xl expression in the hippocampus. Results: Compared to the control group,the expression of Bcl-xl in CA3 region of the hippocampus and dendate gyrus(DG)of the rats was decreased morphologically. With the computerized image analysis, the gray degree increased significantly (P<0.01) and the changes showed the same results after the poststress. Conclusion: Chronic stress decreased the expression of the Bcl-xl in CA3 region and DG in the hippocampus of the rats and the changes showed the same results after the poststress.

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