中国临床心理学杂志

新生期孤养对成年大鼠行为与5-羟色胺转运体表达的影响

单位：1. 暨南大学医学院广东广州 510632
2. 广东金融学院劳经系广东广州 510521
3. 复旦大学社会学博士后流动站

分类号：R395.1

出版年,卷(期):页码：2010,18(4):411-414

摘要：

目的:探讨新生期孤养(neonatal isolation,NI)对成年大鼠行为与5-羟色胺转运体(5-HTT)表达的影响。方法:新生的SD大鼠随机分为正常对照(non-handled,NH)组与新生期孤养组,新生期孤养组在出生后2-14d每天孤养4h,断乳后常规饲养。12w龄时,进行旷场、高架十字迷宫和强迫游泳试验,酶联免疫法检测血浆促肾上腺皮质激素与皮质酮、海马与下丘脑5-HT的含量,原位杂交法观察中缝核5-HTTmRNA的表达。结果:高架十字迷宫试验中新生期孤养组大鼠出入开放臂的总次数与进入开放臂的比率显著减少;强迫游泳试验中新生期孤养组大鼠悬浮时间显著增多(P<0.05)。新生期孤养组大鼠血浆促肾上腺皮质激素与皮质酮显著升高,海马中5-HT含量显著减少,中缝核5-HTTmRNA表达显著增强(P<0.05或P<0.01)。结论:新生期孤养可导致大鼠在成年时出现抑郁和焦虑样行为,可能与下丘脑-垂体-肾上腺轴功能亢进、中枢5-HT功能低下有关。

Objective:To investigate the long-term effects of neonatal isolation(NI) on behavior and expression of serotonin reuptake transporter(5-HTT) in adult rats.Methods:Sprague-Dawley pup was isolated from dam,nest and littermates daily for 4 hours from post-natal day(PND) 2 to PND14(NI) or undisturbed(non-handled,NH),and subjected to behavioral sessions for open-field test,elevated plus maze test and forced swim at 12 weeks after birth.The contents of adrenocorticotrophic hormone(ACTH) and corticosterone(Cort) in plasma were examined,as well as the contents of serotonin(5-HT) in the hippocampus and hypothalamus by Enzyme-linked immunosorbent assay.5-HTT mRNA levels in the dorsal raphe nucleus were observed in situ hybridization.Results:Total entries and percent of entries to open arms of elevated plus maze decreased and immobility duration in swim test increased in NI rats compared with NH rats(P<0.05).Compared with NH rats,the contents of plasma ACTH and Cort increased while the hippocampus contents of 5-HT decreased and the raphe expression of 5-HTT mRNA increased in NI rats(P<0.05).Conclusion:The results suggest that NI may result in the development of depression-/anxiety-like behaviors in adult rats,in which the long-term alterations in hypothalamic-pituitary-adrenal axis and 5-HTergic neurotransmission may take a role.

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