中国临床心理学杂志

慢性束缚应激对小鼠毛囊细胞生长周期的影响

单位：1. 苏州大学基础医学与生物科学学院江苏苏州 215123
2. 河北省衡水市东亚百殿风医院河北衡水 053000

分类号：R395.1

出版年,卷(期):页码：2012,20(6):769-772

摘要：

目的:探讨慢性束缚应激诱导的皮肤氧化应激对小鼠毛囊生长周期的影响。方法:以慢性束缚法建立小鼠心理应激模型,观察行为学和皮肤组织形态学改变,检测血浆皮质醇水平,皮肤组织中硫代巴比妥酸反应产物(TBARS)水平,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力。结果:与控制组相比,束缚组小鼠运动距离显著减少(P<0.05),血浆皮质醇和皮肤TBARS水平显著上升(P<0.05,P<0.001),SOD、GSH-Px活力显著降低(P<0.05,P<0.001),毛囊生长期明显延迟;束缚加抗氧化剂组小鼠,皮肤组织TBARS水平、GSH-Px活力与控制组无明显差异,可部分逆转毛囊生长期延迟。结论:慢性束缚应激引起的皮肤组织氧化应激导致小鼠毛囊生长期延迟。

Objective: To investigate the effects of oxidative stress induced by chronic restraint stress on hair cycle of mouse.Methods: Chronic restraint stress was used to establish a psychological stress model in mouse,to observe the change of behavior and skin tissue morphology,and detect the cortisol levels in plasma,thiobarbituric acid reactive substances(TBARS) levels,superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activity in skin.Results: Compared with the control group,mouse movement distance of restraint group decreased significantly(P<0.05),plasma cortisol and skin TBARS levels increased significantly(P<0.05,P<0.001),SOD and GSH-Px activity decreased significantly(P<0.05,P<0.001),anagen stage was significantly delayed;Compared with the control group,TBARS levels and GSH-Px activity had no significant difference in mouse of restraint added anti-oxidants group,delay of anagen was partly reversed.Conclusion: The oxidative stress induced by chronic restraint stress of skin tissue delayed anagen in hair cycle of mouse.

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