中国临床心理学杂志

慢性应激对不同月龄大鼠前额叶皮质、纹状体脑源性神经营养因子表达的影响

单位：1. 山东大学医学院山东济南 250012
2. 青岛妇幼医院山东青岛 266011
3. 中国人民公安大学北京 100038

分类号：R395.1

出版年,卷(期):页码：2012,20(6):765-768

摘要：

目的:观察慢性应激对不同月龄大鼠行为、前额叶皮质、纹状体脑源性神经营养因子(BDNF)表达的影响。方法:采用慢性轻度不可预知性应激建立动物模型,观察2月龄和15月龄48只Wistar大鼠的行为学、前额叶皮层和纹状体细胞形态学及BDNF的表达。结果:与对照组相比,青年应激大鼠水平运动、直立次数均明显减少,这一变化在老年应激组更明显。应激组糖水偏爱百分比显著低于对照组,并持续到应激结束后一周;老年应激组的糖水偏爱百分比低于青年组。应激组前额叶皮层和纹状体神经细胞呈程度不同的损伤现象,且老年应激组的损伤较青年组严重。应激组前额叶皮质和纹状体BDNF表达明显下调,老年应激组下调明显。应激后一周,青年应激组BDNF表达有一定程度的升高,但仍显著低于对照组。结论:慢性轻度不可预知性应激引起老年大鼠明显的行为学改变,提示老年大鼠对应激的反应性和耐受性降低。应激导致前额叶皮层和纹状体细胞的变性、萎缩和死亡及BDNF表达下降,这一变化在老年组更为明显。

Objective: To study the chronic stress effects on the behavior and BDNF expression in PFC and caudate putamen in different aged rats.Methods: Chronic unpredictable mild stress(CUMS) was used to build 2 month and 15 month old Wistar rats depressive animal model,and to observe the behavior in open field test,sucrose preference,morphologic changes and BDNF expression in PFC and caudate putamen.Results: Compared with the control groups,the young stress groups displayed less square crossing and vertical movement,and the aged stress animals had more remarkable changes in those behavior.The young stress rats showed obviously decreased tendency in sucrose preference compared with the control groups.Meanwhile,the aged stress group exhibited obvious decreases of sucrose preference after stress,and persisted 1 week after stress than the young stress group.The young stress groups displayed obviously morphologic damage in PFC and caudate putamen,the aged stress group showed more cell atrophy and cellular necrosis compared with the young stress group.The young stress rats had significant lower BDNF expression in PFC and caudate putamen than the control groups;the aged stress rats showed even lower BDNF expression than the young stress rats at every testing time.Conclusion: CUMS induces aged rats to more serious abnormal behavior than young animals,indicating aging is one of the important influence factors in stress.The aged rats have more serious cell atrophy and cellular necrosis than the young rats under CUMS.Stress induces significant reduction of BDNF expression in PFC and caudate putamen.The aged stress rats have much lower BDNF expression than the young rats.

基金项目：

山东省自然基金(ZR2011CM015)

作者简介：

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