中国临床心理学杂志

慢性天敌应激诱发吗啡行为敏感化动物模型的建立

作者：魏曙光¹ 张月娟²

单位：1. 河南师范大学教育科学学院河南新乡 453007
2. 武警后勤学院军事心理学教研室天津 300162

分类号：R395.1

出版年,卷(期):页码：2012,20(5):610-612

摘要：

目的:建立慢性天敌应激诱发吗啡行为敏感化动物模型。方法:16只大鼠随机分为慢性天敌应激组、对照组。记录最后一次应激结束24h后大鼠在行为检测箱中20 min的水平运动量,之后急性吗啡给药立即放入检测箱继续检测120 min内的水平运动量。结果:最后一次应激结束24h后应激组大鼠水平活动量低于对照组大鼠的水平活动量,但没有达到统计学显著水平。急性吗啡给药后应激组大鼠的水平运动量显著高于对照组。结论:慢性天敌应激可以增强吗啡诱发的运动反应,表明慢性天敌应激模型可以作为研究慢性应激诱发药物成瘾易感性的动物模型。

Objective: To establish an animal model of chronic predator stress-induced behavioral sensitization to morphine. Methods: 16 rats were randomly divided into 2 groups: chronic stressed and controlled group. Locomotor activity was assessed 24h after the last stress in an activity recording system for 20 minutes. After acute morphine administration, locomotor activity was recorded for 120 minutes. Results: Chronic predator stressed rats showed less locomotor activity responding compared with controlled rats 24h after the last stress, but the difference was not significant statistically.Chronic predator stressed rats showed enhanced locomotor activity compared with controlled rats after acute morphine administration. Conclusion: Chronic predator stress can enhance the locomotor response to morphine in rats, which suggests chronic predator stress model could be an important model for the research in the vulnerability of drug addiction.

基金项目：

河南师范大学博士启动基金(01106500106)

作者简介：

参考文献：

[1] 张瑞岭，李凌江，郝伟. 应激易化成瘾及复吸的生物学机制. 中国临床心理学杂志，2000,8(1)：62-64
[2] Cruz FC, Le觔o RM, Marin MT, et al. Stress-induced rein-statement of amphetamine -conditioned place preference and changes in tyrosine hydroxylase in the nucleus accumbens in adolescent rats. Pharmacology Biochemistry and Behavior, 2010, 96(2): 160-165
[3] Leao RM, Cruz FC, Planeta CS. Exposure to acute restraint stress reinstates nicotine induced place preference in rats. Behavioral Pharmacology, 2009, 20(1): 109-113
[4] Covington III HE, Miczek KA. Intense cocaine self-administration after episodic social defeat stress, but not afteraggressive behavior: dissociation from corticosterone activation. Psychopharmacology, 2005, 183(3): 331-340
[5] Quadros IM, Miczek KA. Two modes of intense cocainebingeing: increased persistence after social defeat stress and increased rate of intake due to extended access conditions in rats. Psychopharmacology, 2009, 206(1): 109-120
[6] Robinson TE, Berridge KC. Addiction. Annual Review of Psychology, 2003, 54: 25-53
[7] Robinson TE, Berridge KC. The incentive sensitization theory of addiction: some current issues. Philosophical Transactions of the Royal Society B: Biological Sciences,2008, 363: 3137-3146
[8] Fleshner M, Deak T, Spencer RL, et al. A long-term in-crease in basal levels of corticosterone and a decrease in corticosteroid-binding globulin after acute stressor expo-sure. Endocrinology, 1995, 136(12): 5336-5342
[9] 魏曙光，张月娟. 青龄大鼠和成龄大鼠在吗啡条件性位置偏爱和行为敏感化效应上的差异. 中华行为医学与脑科学杂志，2009,18(11)：979-981
[10] Cruz FC, Marin MT, Le觔o RM, et al. Behavioral and neuroendocrine effects of the exposure to chronic restraint orvariable stress in early adolescent rats. International Jour-nal of Developmental Neuroscience, 2012, 30: 19-23
[11] 赵虎. 天敌应激对大鼠海马神经元的损害作用及海马类固醇激素受体系统对精神应激反应的调节效应研究. 汕头大学博士论文，2003
[12] Aranjo APN, Delucia R, Scavone C, et al. Repeated predictable or unpredictable stress: effects on cocaine induced locomotion and cyclic AMP-dependent protein kinase activity. Behavioural Brain Research, 2003, 139: 75-81
[13] Stohr T, Almeida OF, Landgraf R, Shippensburg TS, et al. Stress- and corticosteroid-induced modulation of the locomotor response to morphine in rats. Behavioral Brain Research, 1999, 103: 85-93

服务与反馈：

【文章下载】【加入收藏】